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Cannabis Concentrate Sample on Raptor ARC-18 1.8 μm by UHPLC-UV

PeakstR (min)
1.Δ9-Tetrahydrocannabinol (Δ9-THC)2.49
image
LC_GN0592
ColumnRaptor ARC-18 (cat.# 931421E)
Dimensions:100 mm x 3.0 mm ID
Particle Size:1.8 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter (cat.# 25809)
Temp.:30 °C
Sample
Diluent:25:75 Water:methanol
Inj. Vol.:2 µL
Mobile Phase
A:Water, 5 mM ammonium formate, 0.1% formic acid
B:Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.001.02575
4.001.02575
DetectorUV/Vis @ 228 nm
InstrumentUHPLC
Notes20 mg of concentrate was weighed into a 50 mL centrifuge tube. 10 mL of ethanol was added prior to vortexing (5 seconds) and sonicating (5 minutes) over a total of three cycles. The sample was then centrifuged for 5 minutes at 3000 rpm. The supernatant was diluted 50-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.
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cannabinoids

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potency

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