Mycotoxins (STD 1 - 2 ng/g) in MCT Oil on Raptor Biphenyl by LC-MS/MS

PeakstR (min)Precursor IonProduct Ion 1Product Ion 2
1.Aflatoxin G2-13C171.340348.3330.3-
2.Aflatoxin G21.344331.2189.3115.2
3.Aflatoxin G1-13C171.576346.3257.3-
4.Aflatoxin G11.579329.2243.2215.3
5.Aflatoxin B2-13C171.707332.3303.3-
6.Aflatoxin B21.711315.3287.2243.3
7.Ochratoxin A1.824404.3239.1358.3
8.Ochratoxin A-13C201.825424.3250.2-
9.Aflatoxin B11.946313.2241.2128.2
10.Aflatoxin B1-13C171.948330.3301.4-
ColumnRaptor Biphenyl (cat.# 9309A52)
Dimensions:50 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:35 °C
Diluent:45:55 Water:Methanol
Conc.:2 ng/g
Inj. Vol.:5 µL
Mobile Phase
A:Water, 2 mM ammonium formate, 0.1% formic acid
B:Methanol, 2 mM ammonium formate, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
Ion Mode:ESI+
NotesA working calibration solution containing Aflatoxin B1, B2, G1, and G2 and Ochratoxin A was prepared at 50.0 ng/mL in methanol. 0.25 g of MCT oil was spiked to 2 ng/g using 10 µL of the working calibration solution. A working internal standard was prepared using 13C labeled analogs at a concentration of 250 ng/mL in methanol. 10 µL of the working internal standard was aliquoted into the sample followed by vortexing for 10 seconds at 3,000 rpm. 1 mL of 45:55 H2O:MeOH was added to the sample. The sample was vortexed for 30 seconds at 3,000 rpm. The sample was then centrifuged at 3,000 xg for 5 min. at 10 °C. 750 µL of the supernatant was transferred to a conditioned (1 mL 45:55 water:methanol) Resprep bonded reversed phase SPE cartridge (Restek cat.# 26030). The sample was pulled through under vacuum into an autosampler vial for LC-MS/MS analysis.
Download PDF

relativo alle ricerche nella libreria dei cromatogrammi




Consigli per la stampa! Per stampare cromatogrammi su tutta la pagina, cambiare le impostazioni di stampa nella finestra di stampa del vostro software PDF reader: 1)"adatta" o "adatta alla pagina", 2)"panorama" per cromatogrammi grandi o "ritratto" per cromatogrammi alti.