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22 Acylcarnitines and 13 Amino acids (Endogenous) in Dried Blood Spots with 16 Deuterated Internal Standards on Raptor HILIC-Si EXP Guard Cartridge Column by LC-MS/MS
| Peaks | tR (min) | Precursor Ion | Product Ion | |
|---|---|---|---|---|
| 1. | Phenylalanine | 0.20 | 166.0 | 120.1 |
| 2. | Leucine | 0.21 | 132.1 | 86.0 |
| 3. | Leucine-d3 | 0.21 | 135.2 | 89.1 |
| 4. | Isoleucine | 0.21 | 132.1 | 86.1 |
| 5. | Tyrosine | 0.22 | 182.1 | 91.0 |
| 6. | Methionine | 0.24 | 150.1 | 56.1 |
| 7. | Methionine-d3 | 0.24 | 153.2 | 107.1 |
| 8. | Valine | 0.27 | 118.1 | 72.0 |
| 9. | C20-Eicosanoyl-L-carnitine | 0.27 | 456.4 | 85.1 |
| 10. | C18-Stearoyl-L-carnitine | 0.28 | 428.3 | 85.1 |
| 11. | C18-Stearoyl-L-carnitine-d3 | 0.29 | 431.4 | 85.1 |
| 12. | C18:1 Oleoyl-L-carnitine | 0.29 | 426.4 | 85.1 |
| 13. | C18:2 Linoleoyl-L-carnitine | 0.30 | 424.3 | 85.1 |
| 14. | C16-Palmitoyl-L-carnitine | 0.30 | 400.3 | 85.1 |
| 15. | C16-Palmitoyl-L-carnitine-d3 | 0.31 | 403.3 | 85.1 |
| 16. | C16:1 Palmitolelyl-L-carnitine | 0.31 | 398.3 | 85.1 |
| 17. | C14-Myristoyl-L-carnitine | 0.32 | 372.3 | 85.1 |
| 18. | C14-Myristoyl-L-carnitine-d3 | 0.33 | 375.3 | 85.1 |
| 19. | C14:1 Tetradecenoyl-L-carnitine | 0.33 | 370.3 | 85.1 |
| 20. | C14:2-Tetradecadienoyl-L-carnitine | 0.33 | 368.3 | 85.1 |
| 21. | Proline | 0.33 | 116.0 | 70.1 |
| 22. | C12-Lauroyl-L-carnitine | 0.35 | 344.3 | 85.1 |
| 23. | C12-Lauroyl-L-carnitine-d3 | 0.35 | 347.3 | 85.1 |
| 24. | Alanine | 0.36 | 90.1 | 44.1 |
| 25. | Alanine-d4 | 0.35 | 94.1 | 48.1 |
| 26. | C10-Decanoyl-L-carnitine | 0.38 | 316.3 | 85.1 |
| 27. | C10-Decanoyl-L-carnitine-d3 | 0.39 | 319.2 | 85.1 |
| 28. | C8-Octanoyl-L-carnitine | 0.43 | 288.3 | 85.1 |
| 29. | C8-Octanoyl-L-carnitine-d3 | 0.43 | 291.2 | 85.1 |
| 30. | C7-Heptanoyl-L-carnitine | 0.45 | 274.2 | 85.1 |
| 31. | C6-Hexanoyl-L-carnitine-d3 | 0.46 | 263.2 | 85.1 |
| 32. | C6-Hexanoyl-L-carnitine | 0.47 | 260.2 | 85.1 |
| 33. | Glutamine | 0.45 | 147.1 | 84.1 |
| 34. | C5-Valeryl-L-carnitine | 0.48 | 246.2 | 85.1 |
| 35. | C5-Valeryl-L-carnitine-d3 | 0.49 | 249.1 | 85.1 |
| 36. | C5-Isovaleryl-L-carnitine | 0.49 | 246.1 | 85.1 |
| 37. | C5-Isovaleryl-L-carnitine-d3 | 0.49 | 249.2 | 85.1 |
| 38. | 2-Methylbutyryl-L-carnitine | 0.49 | 246.2 | 85.1 |
| 39. | C5:1-Tiglyl-L-carnitine | 0.50 | 244.2 | 85.1 |
| 40. | C4-Butyryl-L-carnitine | 0.51 | 232.2 | 85.1 |
| 41. | C4-Butyryl-L-carnitine-d3 | 0.51 | 235.2 | 85.1 |
| 42. | C4-Isobutyryl-L-carnitine | 0.51 | 232.1 | 85.1 |
| 43. | C4-Isobutyryl-L-carnitine-d3 | 0.51 | 235.1 | 85.1 |
| 44. | Citrulline | 0.51 | 176.1 | 113.1 |
| 45. | Glutamic acid | 0.55 | 148.1 | 83.9 |
| 46. | C3-Propionyl-L-carnitine | 0.54 | 218.1 | 85.1 |
| 47. | C3-Propionyl-L-carnitine-d3 | 0.54 | 221.2 | 85.1 |
| 48. | C2-Acetyl-L-carnitine | 0.56 | 204.1 | 85.1 |
| 49. | C2-Acetyl-L-carnitine-d3 | 0.56 | 207.1 | 85.1 |
| 50. | Arginine | 0.66 | 175.2 | 70.1 |
| 51. | Ornithine | 0.69 | 133.1 | 70.1 |
All analytes were present endogenously at varying concentrations in whole blood, except the deuterated internal standards.
LC_CF0759
| Column | Raptor HILIC-Si EXP guard cartridge column (cat.# 9310A0252) | ||||||||||||||||||||
| Dimensions: | 5 mm x 2.1 mm ID | ||||||||||||||||||||
| Particle Size: | 2.7 µm | ||||||||||||||||||||
| Temp.: | 45 °C | ||||||||||||||||||||
| Sample | |||||||||||||||||||||
| Diluent: | 85:15 Acetonitrile:water (v/v) | ||||||||||||||||||||
| Conc.: | Endogenous levels | ||||||||||||||||||||
| Inj. Vol.: | 2.0 µL | ||||||||||||||||||||
| Mobile Phase | |||||||||||||||||||||
| A: | 30 mM Ammonium formate in water | ||||||||||||||||||||
| B: | Acetonitrile | ||||||||||||||||||||
| |||||||||||||||||||||
| Detector | MS/MS | ||||||||||||||||||||
| Ion Source: | Electrospray | ||||||||||||||||||||
| Ion Mode: | ESI+ | ||||||||||||||||||||
| Instrument | UHPLC | ||||||||||||||||||||
| Notes | 50 µL of whole blood was spotted on to Whatman 903 neonatal protein saver cards, which were then dried for 1 hour at room temperature. A 3.0 mm disk (∼3.0 µL whole blood) was punched out of the dried spot and into a 2.0 mL Eppendorf tube. 200 µL of 85:15 acetonitrile:water (v/v) that was fortified with known concentrations of stable isotope-labeled internal standards was added, and then the sample was vortexed and incubated for 20 minutes at room temperature on a microplate shaker at a speed of 400 rpm. The sample was then centrifuged for 10 minutes at 4000 rpm, and 150 µL of the supernatant was filtered using a Thomson SINGLE StEP Nano filter vial (cat.# 25882) prior to LC-MS/MS analysis. |
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