Innovative Chromatography Products
www.restek.it

Libreria cromatogrammi ricercabili Re...

LC_CF0686

Questo cromatogramma potrebbe contenere analiti che sono sinonimi dei vostri parametri di ricerca.

Immunosuppressive Drugs in Blood on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
1.	Ascomycin	1.45	10	809.53	756.50
2.	Tacrolimus	1.47	10	821.61	768.51
3.	Sirolimus	1.64	10	931.63	864.57
4.	Everolimus	1.69	10	975.68	908.62
5.	Cyclosporin A	1.83	10	1219.83	1202.87
6.	Cyclosporin D	1.89	100	1233.91	1216.88

LC_CF0686

Column

Raptor Biphenyl (cat.# 9309A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

70 °C

Inj. Vol.:

5 µL

Mobile Phase

0.05% Formic acid, 5 mM ammonium formate in water

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	40	60
2.00	0.5	0	100
2.01	0.5	40	60
3.00	0.5	40	60

Max Pressure:

305 bar

Detector

MS/MS

Ion Mode:

ESI+

Mode:

MRM

Instrument

UHPLC

Notes

Sample Preparation Procedure:
Human whole blood was fortified with 4 immunosuppressive drugs at 10 ng/mL. For quantitation, cyclosporin D was used as the internal standard for cyclosporin A and ascomycin was used as the internal standard for tacrolimus, sirolimus, and everolimus. The blood sample (100 μL) was mixed with 200 μL of precipitation solution (1:4 v/v 0.2 M ZnSO₄:methanol) containing 50 ng/mL of cyclosporin D and 5 ng/mL of ascomycin. The mixture was vortexed for 20 seconds at 3,000 rpm and then centrifuged for 10 minutes at 4,300 rpm. The supernatant was directly injected (5 μL) for analysis.

Download PDF

relativo alle ricerche nella libreria dei cromatogrammi

cyclosporin

tacrolimus

sirolimus

everolimus

raptor biphenyl