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GC Potency Analysis for Marijuana on Rxi-5Sil MS
Peaks | tR (min) | Conc. (wt.%) | |
---|---|---|---|
1. | CBD![]() | 4.035 | 0.0 |
2. | THC![]() | 4.524 | 3.6 |
3. | CBN![]() | 4.840 | 0.3 |

Column | Rxi-5Sil MS, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13620) |
Injection | |
Inj. Vol.: | 1 µL split (split ratio 20:1) |
Liner: | Premium 4.0 mm ID single taper inlet liner w/wool (cat.# 23303.5) |
Inj. Temp.: | 250 °C |
Oven | |
Oven Temp.: | 200 °C (hold 0 min) to 300 °C at 15 °C/min (hold 0 min) |
Carrier Gas | H2, constant pressure (7 psi, 48.3 kPa) |
Temp.: | 200 °C |
Dead Time: | 0.6 min @ 200 °C |
Detector | FID @ 300 °C |
Make-up Gas Flow Rate: | 45 mL/min |
Make-up Gas Type: | N2 |
Instrument | HP5890 GC |
Notes | Blue trace = cannabinoids standard (cat.# 34014) diluted to 100 µg/mL in isopropyl alcohol. Red trace = extracted marijuana sample Sample extraction: Weigh 0.2 g of sample into a 40 mL VOA vial, add 40 mL of isopropyl alcohol, shake for 5 minutes, and allow sample to settle. Quantification: Potency values (weight%) were based on a 1-point standard curve using the standard show above. |
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