GC Potency Analysis for Marijuana on Rxi-5Sil MS

PeakstR (min)Conc.
ColumnRxi-5Sil MS, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13620)
Inj. Vol.:1 µL split (split ratio 20:1)
Liner:Premium 4.0 mm ID single taper inlet liner w/wool (cat.# 23303.5)
Inj. Temp.:250 °C
Oven Temp.:200 °C (hold 0 min) to 300 °C at 15 °C/min (hold 0 min)
Carrier GasH2, constant pressure (7 psi, 48.3 kPa)
Temp.:200 °C
Dead Time:0.6 min @ 200 °C
DetectorFID @ 300 °C
Make-up Gas Flow Rate:45 mL/min
Make-up Gas Type:N2
InstrumentHP5890 GC
NotesBlue trace = cannabinoids standard (cat.# 34014) diluted to 100 µg/mL in isopropyl alcohol.
Red trace = extracted marijuana sample

Sample extraction: Weigh 0.2 g of sample into a 40 mL VOA vial, add 40 mL of isopropyl alcohol, shake for 5 minutes, and allow sample to settle.

Quantification: Potency values (weight%) were based on a 1-point standard curve using the standard show above.
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