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QuEChERS Extracts of Tylenol Spiked with Anisoles and TBA on Rxi-5Sil MS

ColumnRxi-5Sil MS, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13623)
SampleQuEChERS extracts of Tylenol spiked with TBA and anisoles
Inj. Vol.:1 µL splitless (hold 1 min)
Liner:Gooseneck Splitless (4mm) w/Wool (cat.# 22405)
Inj. Temp.:250 °C
Purge Flow:40 mL/min
Oven Temp.:90 °C (hold 1 min) to 310 °C at 4 °C/min (hold 2 min)
Carrier GasHe, constant flow
Flow Rate:1.2 mL/min
Linear Velocity:40 cm/sec
Transfer Line Temp.:290 °C
Analyzer Type:TOF
Source Temp.:225 °C
Electron Energy:70 eV
Mass Defect:-20 mu/100 u
Solvent Delay Time:2 min
Tune Type:PFTBA
Ionization Mode:EI
Acquisition Range:45 to 550 amu
Spectral Acquisition Rate:5 spectra/sec
InstrumentLECO Pegasus 4D GCxGC-TOFMS
NotesTotal ion chromatogram (pink) of QuEChERS extracts of Tylenol spiked with anisoles and TBA, and extracted ion chromatogram (blue, 265x50) of pentachloroanisole, which was located by an automated peak find routine underneath the large acetaminophen peak. No dSPE was performed on this sample; see Applications Note GNAN1263 for complete sample fortification and extraction details.
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