Method 505: Analysis of Organohalide Pesticides and Commercial PCBs in Water by Microextraction and GC

by Microextraction and Gas Chromatography

Seven EPA Methods on One Column With the advent of modern agriculture and its vast selection of chemical pest control measures, the farming community has made significant increases in productivity and efficiency. Crop yield per acre is extremely high, due in part to the role of pesticides and herbicides in mitigating the devastating effects of many plant and insect pests. However, the use of these chemicals can have drawbacks, including surface and ground water contamination. EPA Method 505 is used for organohalide pesticide analysis in drinking and ground water. The Rtx-CLPesticides and Rtx-CLPesticides2 column set works well for the analysis of organohalide compounds because the columns are highly selective for analytes that contain electronegative substituents. The optimized parallel dual-column method shown here provides fast analysis times, which improves sample throughput.

Vendor Column Pair Analysis Time (min) Coelutions Restek Advantage
Restek Rtx-CLPesticides /
Rtx-CLPesticides2
18 / 18.5 1 / 1
  • All compounds resolved on at least one column.
Competitor A column 1 / column 2 14 / 14 0 / 1
Competitor B column 1 / column 2 35 / 36 1 / 2
Comparison based on published competitor data. All columns tested were 0.32 mm ID.

Organohalide pesticide analysis (Method 505) on Rtx-CLPesticides and Rtx-CLPesticides2 columns. View larger

Peaks
1.Hexachlorocyclopentadiene
2.Hexachlorobenzene
3.Simazine
4.Atrazine
5.γ-BHC
6.Heptachlor
7.Alachlor
8.Aldrin
Peaks
9.Heptachlor epoxide
10.trans-Chlordane*
11.trans-Nonachlor
12.cis-Chlordane*
13.Dieldrin
14.Endrin
15.cis-Nonachlor
16.Methoxychlor
* For information regarding the nomenclature used for cis-chlordane and trans-chlordane, visit www.restek.com/chlordane-notice
Pesticides of U.S. EPA Method 505 on Rtx-CLPesticides and Rtx-CLPesticides2
GC_EV01004
ColumnsRtx-CLPesticides 30 m, 0.32 mm ID, 0.32 µm (cat.# 11141)
and Rtx-CLPesticides2 30 m, 0.32 mm ID, 0.25 µm (cat.# 11324)
using Rxi guard column 5 m, 0.32 mm ID (cat.# 10039)
with deactivated universal “Y” Press-Tight connector (cat.# 20405-261)
Sample200 ng/mL 505 organohalide pesticide mix (cat.# 32024)
4.2 µg/mL simazine (cat.# 32236)
4.2 µg/mL atrazine (cat.# 32208)
Diluent:Methanol
Injection
Inj. Vol.:2 µL splitless (hold 0.75 min)
Liner:Cyclo double taper (4 mm) (cat.# 20896)
Inj. Temp.:250 °C
Oven
Oven Temp.:90 °C (hold 1 min) to 310 °C at 10 °C/min (hold 5 min)
Carrier GasHe, constant flow
Linear Velocity:40 cm/sec
DetectorMicro-ECD @ 325 °C
InstrumentAgilent/HP6890 GC
NotesThis chromatogram was obtained using an Agilent micro-ECD. To obtain comparable results, you will need to employ a micro-ECD in addition to confirmational dual columns connected to a 5-meter guard column using a “Y” Press-Tight connector.

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