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HPLC Analysis of Glucosinolates in Vegetable Extracts without Ion Pairing Using an Ultra Aqueous C18 Column

Glucosinolates with hydrophilic functional groups are very difficult to retain by conventional reversed phase HPLC, and when the functional groups in a sample are diverse, polarity can differ widely, complicating the problem. Until now, reversed phase HPLC with an ion pairing reagent has been the inconvenient, but necessary, approach to these analyses. This 2-page note describes a column and conditions for analyses of glucosinolates, without ion pairing.

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