MSACL 2019 U.S.

11th Annual Conference & Exhibits of The Association for Mass Spectrometry:
Applications to the Clinical Laboratory, Inc.

Restek at MSACL U.S.
Restek at MSACL U.S.

CORPORATE WORKSHOP

Coated Blade Spray-Mass Spectrometry: A Novel Strategy for Rapid Screening and Quantitation
German Gomez1, Kara Lynch2 (presenter)
1. Restek Corporation, 2. University of California, San Francisco
For more information, email German Gomez.
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Coated Blade Spray (CBS) is a novel sampling/sample-preparation technology that can be directly interfaced to Mass Spectrometry (MS) instrumentation for rapid screening and quantitation of small molecules present in complex matrices. A comparative study between a Point of Care-CUP test and CBS-MS/MS for drug screening in urine samples of chronic pain patients showed that, unlike the POC-CUP test, where determination of each class of drugs requires a separate assay, the CBS-MS method is able to provide data for all selected drugs and metabolites in a single analytical run. The University of Waterloo and the University of Toronto are now collaborating on quantitating four immunosuppressive drugs (i.e., cyclosporine A, tacrolimus, everolimus, and sirolimus) via CBS-MS/MS in blood samples of Canadian patients. A comparative study between CBS-MS/MS and the automated immunoassay showed good agreement between both methods with observed differences consistent with those formerly reported in literature.

TECHNICAL POSTERS

Simultaneous Quantitation of Tacrolimus, Sirolimus, Everolimus, and Cyclosporine-A in Whole Blood via Coated Blade Spray-Mass Spectrometry
German Gomez (presenter)
Restek Corporation
For more information, email German Gomez.
Download a PDF of the full presentation.
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Coated Blade Spray (CBS) is a technology that combines sample preparation and direct coupling to mass spectrometry (MS) on a single device. Herein, CBS-MS is presented as a novel technology for the rapid and concurrent determination of commonly prescribed immunosuppressive drugs (ISDs) in whole blood: tacrolimus (TAC), cyclosporine-A (CycA), sirolimus (SIR), and everolimus (EVR). The assay was linear within the evaluated range of concentrations (0.1 - 100 ng/mL for EVR, SIR, and TAC; 1 - 1000 ng/mL for CycA). The limits of quantification were determined to be 2.5 ng/mL for CycA and below 1 ng/mL for EVR, SIR, and TAC.

A Novel Solution for Vitamin K1 and K2 Analysis in Human Plasma by LC-MS/MS
Dan Li (presenter)
Restek Corporation
For more information, email Dan Li.
Download a PDF of the full presentation.
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Vitamin K is a group of fat-soluble vitamins divided into vitamin K1 (one compound, phylloquinone) and K2 (a group of compounds, menaquinones). As an interest for their biological action in extra-hepatic tissues is increasing, an accurate and simple measurement of vitamin K status remains a critical issue for both clinical research and diagnostics. There is a high degree of analytical challenges in vitamin K analysis, as it is the most lipophilic and least abundant of the fat-soluble vitamins. In this study, a simple and fast plasma sample preparation procedure was developed using phospholipid removal in combination with a chromatographic analysis using a Raptor Biphenyl column. The method provides a novel solution towards vitamin K research and high-throughput clinical diagnosis.

Analysis of D and L-Amphetamines Using Reversed-Phase LC-MS/MS
Frances Carroll (presenter)
Restek Corporation
For more information, email Frances Carroll.
Download a PDF of the full presentation.
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Amphetamine and methamphetamine are psychostimulant drugs and occur as two enantiomers, dextrorotary and levorotary, as a result of their chiral center. The dextro-methamphetamine (d-isomer) form is highly abused and is typically found in illicit preparations. However, detection of abuse is complicated because consumption of over-the-counter and prescription medications may yield positive results if the analytical method used cannot distinguish between the enantiomers. Our method employs a simple precolumn derivatization followed by dilution and results in a selective, specific analysis of d- and l-amphetamine and methamphetamine enantiomers using a 7-minute, reversed-phase gradient on a C18 column.

Quantitative Analysis of 58 Antipsychotics and Antidepressants in Human Urine by LC-MS/MS
Shane Stevens (presenter)
Restek Corporation
For more information, email Shane Stevens.
Download a PDF of the full presentation.
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Human mental health disorders have become modern-day living diseases and contribute significantly to worldwide morbidity and mortality. This is reflected in fast growing numbers of antipsychotics (APs) and antidepressants (ADs) entering the market and the rising rate of prescription. It has been reported that the patient’s adherence to prescribed APs is quite low, and thus new clinical recommendations have been released regarding when and how to use urine testing procedures to help monitor adherence. A comprehensive method was developed for simultaneous quantification of 58 AP/AD drugs and metabolites using the combination of a simple urine hydrolysis procedure and a fast 5.5-minute chromatographic method with special emphasis given to the separation of isobaric compounds and the reduction of carryover.

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