LC Potency Testing for Medical Marijuana on Ultra Aqueous C18

PeakstR (min)Conc.
(wt.%)
1.CBD2.5070.1
2.CBN3.6320.0
3.THC3.9770.5
4.THCA5.3644.5
image
LC_GN0530
ColumnUltra Aqueous C18 (cat.# 9178312)
Dimensions:100 mm x 2.1 mm ID
Particle Size:3 µm
Pore Size:100 Å
Temp.:30 °C
Sample
Conc.:
Inj. Vol.:10 µL
Mobile Phase
A:Water + 10 mM potassium phosphate (pH = 2.5)
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.42080
1.00.42080
5.00.4595
6.00.4595
6.10.42080
8.00.42080
DetectorUV/Vis @ 220, 4 nm
Cell Temp:40 °C
InstrumentShimadzu UFLCXR
NotesBlue trace = cannabinoids standards (cat.#s 34014 and 34093) diluted to 100 μg/mL in isopropyl alcohol
Red trace = extracted marijuana sample

Sample extraction: Weigh 0.2 g of sample into a 40 mL VOA vial, add 40 mL of isopropyl alcohol, shake for 5 minutes, and allow sample to settle. Dilute extract 10x with isopropyl alcohol.


Quantification: Potency values (weight%) were based on a 1-point standard curve using the standard show above.
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