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Featured Application: Mycotoxins on Raptor FluoroPhenyl

Highly Selective LC-MS/MS Analysis of Mycotoxins from Multiple Classes

  • Unique column selectivity allows simultaneous determinations of 20 mycotoxins.
  • Complete separation of isobaric compounds that cannot be distinguished by MS.
  • Fast, 9-minute analysis supports high-throughput product inspection testing needs.

Mycotoxins are toxic compounds produced by fungi that can occur naturally on a broad range of agricultural commodities, including produce, grains, animal feeds, and even medical marijuana. They may also resist decomposition by digestion and pasteurization and, as a result, can be found in meats, milk, and eggs as contaminants. Accurate identification of a wide variety of these chemically stable compounds is critical to ensure consumer safety and to satisfy import requirements during international trade. Demand for simultaneous LC-MS/MS analysis of mycotoxins from multiple classes is increasing because it is more efficient than individual analyses and better supports the need for fast, comprehensive determinations that allow correct decisions to be made during product inspection.

The LC-MS/MS analysis of mycotoxins shown here allows 20 mycotoxins from multiple classes to be accurately reported in a fast, 9-minute analysis. Under these conditions the Raptor FluoroPhenyl column’s selectivity allows compounds that are similar in structure and that elute closely together to be adequately resolved. In fact, the Raptor FluoroPhenyl column provides full chromatographic separation of 15-acetyldeoxynivalenol and 3-acetyldeoxynivalenol, isobaric compounds that cannot be resolved on other column phases. Labs performing LC-MS/MS analysis of mycotoxins that require definitive chromatographic identification and quantification of complex analyte lists can benefit from the unique selectivity of a Raptor FluoroPhenyl column.


PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct IonProduct Ion
1.Nivalenol1.04100313.22175.0991.08
2.Patulin1.4250155.0098.9480.98
3.Deoxynivalenol1.5350297.20249.04231.03
4.Fusarenon X2.13100355.20247.10175.08
5.15-Acetyldeoxynivalenol3.07100339.23321.15137.07
6.3-Acetyldeoxynivalenol3.22100339.16231.10213.08
7.Aflatoxin M14.1125329.22273.09229.12
8.Diacetoxyscirpenol4.41100367.24307.14105.07
9.Aflatoxin G24.6725331.16313.00189.01
10.Aflatoxin G14.8725329.13243.00199.74
11.HT-24.9250447.20345.08285.11
12.Aflatoxin B25.2025315.13287.02258.98
13.Aflatoxin B15.3225313.16284.85241.06
14.Citrinin5.3810251.15233.09205.05
15.Fumonisin B15.5625722.46352.25334.23
16.T-25.6350489.24245.07387.12
17.Fumonisin B36.0325706.43336.23318.29
18.Ochratoxin A6.1725404.07238.96357.98
19.Fumonisin B26.3125706.43336.23318.29
20.Zearalenone6.4450319.23283.06187.04
Analysis of Mycotoxins on Raptor FluoroPhenyl by LC-MS/MS
LC_FS0511
ColumnRaptor FluoroPhenyl (cat.# 9319A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor FluoroPhenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9319A0252)
Temp.:30 °C
Sample
Diluent:Water:methanol (80:20)
Conc.:10-100 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:0.3% Formic acid in water
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.48020
7.000.41090
7.010.48020
9.000.48020
Max Pressure:525 bar
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC

Ricerche correlate

LC-MS/MS analysis of mycotoxins